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M9490369.TXT
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1994-09-19
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Document 0369
DOCN M9490369
TI In situ detection of polymerase chain reaction-amplified HIV-1 nucleic
acids in skeletal muscle in patients with myopathy.
DT 9411
AU Seidman R; Peress NS; Nuovo GJ; Department of Pathology, SUNY, Stony
Brook.
SO Mod Pathol. 1994 Apr;7(3):369-75. Unique Identifier : AIDSLINE
MED/94336630
AB The purpose of this study was to determine if the myopathy that commonly
occurs in patients with AIDS is associated with active HIV-1 infection
in the muscle tissues. Seven muscle biopsies from patients infected by
HIV-1 and six controls were tested for HIV-1 DNA and RNA using
polymerase chain reaction in situ hybridization and reverse
transcriptase in situ polymerase chain reaction. HIV-1 DNA was detected
in rare cells in only one case by standard in situ hybridization.
However, after polymerase chain reaction amplification HIV-1 DNA was
detected in many cells in four of seven muscle tissues from patients
with the viral infection and in none of the controls. The number of
cells with detectable provirus in the tissue positive by standard in
situ hybridization increased up to 100-fold after amplification. Most of
the HIV-1 infected cells were macrophages, as determined by colabeling
experiments that were localized mainly in the areas of myocyte necrosis.
Myocyte nuclei that contained amplified HIV-1 nucleic acids were also
noted. Most virally infected cells contained HIV-1 transcripts, which is
consistent with activated infection. The demonstration of many HIV-1
infected macrophages and myocytes in muscle biopsies from HIV-1 infected
patients with myopathy suggests that active viral infection may play a
role in the clinical disease state.
DE Adult Aged DNA, Viral/*ANALYSIS Female Human HIV
Infections/*MICROBIOLOGY HIV-1/*GENETICS Immunoenzyme Techniques In
Situ Hybridization Male Middle Age Muscles/*MICROBIOLOGY/PATHOLOGY
Myositis/*MICROBIOLOGY/PATHOLOGY Polymerase Chain Reaction RNA,
Viral/*ANALYSIS Support, Non-U.S. Gov't JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).